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Type of Document	Dissertation
Author	Temin, Howard M.
URN	etd-10222002-093114
Persistent URL	http://resolver.caltech.edu/CaltechETD:etd-10222002-093114
Title	The interaction of Rous sarcoma virus and cells in vitro
Degree	PhD
Option	Biology
Advisory Committee	Advisor Name Title Unknown Committee Member
Keywords	None
Date of Defense	1960-01-01
Availability	unrestricted
Abstract By the use of new methods for assaying in vitro Rous sarcoma virus (RSV) and Rous sarcoma cells and for isolating the progeny of single particles of RSV, it has been found that the morphology of Rous sarcoma cells is partially controlled by genetic factors in the virus. The influence of the state of the cell before infection, operating directly on the virus-cell complex has been demonstrated. A single particle of RSV is able to initiate infection, but viral genetic factors and genetic and physiological factors in the cell determine whether or not a cell does become infected. Host range mutants of the virus are described. It was shown that there exists a state in which resistant cells become competent to support the growth of RSV. RSV rapidly adsorbs and penetrates into cells. After a 12 hour period new virus appears. The final rate of virus release of 10 focus-forming units per cell per 10 hours is reached about 40 hours after infection. The ability of a cell to produce RSV is transmitted to its progeny as an intracellular event, the number of Rous sarcoma cells doubling every 15 - 20 hours. Immediately after infection the X-ray resistance of the capacity of a cell to release virus is the same as its capacity to divide. By 15 - 20 hours after infection, the resistance of the capacity of the cells to release virus increases about 30 times. Direct observation of isolated cells in microdrops shows that a cell can release virus and then divide. Interference caused by one particle of inactive virus, operating on some intracellular process after penetration of the virus into the cell was found. The relationship between RSV and temperate bacteriophage, the relevance of the findings to the cancer problem, and the relationship between RSV and other animal viruses are discussed.
Files	Filename       Size       Approximate Download Time (Hours:Minutes:Seconds)     28.8 Modem   56K Modem   ISDN (64 Kb)   ISDN (128 Kb)   Higher-speed Access    Temin_hm_1960.pdf 5.10 Mb 00:23:35 00:12:08 00:10:37 00:05:18 00:00:27