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Type of Document	Dissertation
Author	Kastner, Jason
Author's Email Address	kastner AT alumni.caltech.edu
URN	etd-10042002-200303
Persistent URL	http://resolver.caltech.edu/CaltechETD:etd-10042002-200303
Title	Modeling a Hox gene metwork: stochastic simulation with experimental perturbation
Degree	PhD
Option	Applied Mathematics
Advisory Committee	Advisor Name Title Dan Meiron Committee Chair Jerry Solomon Committee Member Joel Franklin Committee Member Niles Pierce Committee Member Scott Fraser Committee Member
Keywords	computational biology model stochastic hox gene network simulation
Date of Defense	2002-09-25
Availability	unrestricted
Abstract The Hox genes show a striking segment specific pattern of expression in a variety of vertebrate embryos, and have been the topic of many experimental analyses. There are now sufficient data to construct a higher-level model for the interaction and regulation of the Hox genes. This thesis presents the results of an investigation into a regulatory network for the early Hox genes. Instead of using conventional differential equation approaches for analyzing the system, a stochastic simulation algorithm has been employed to model the network. The model can track the behavior of each component of a biochemical pathway and produce computerized movies of the time evolution of the system that is a result of the dynamic interplay of these various components. The simulation is able to reproduce key features of the wild-type pattern of gene expression, and in silico experiments yield results similar to their corresponding in vivo experiments. This work shows the utility of using stochastic methods to model biochemical networks and expands the stochastic simulation algorithm methodology to work in multi-cellular systems. In addition, the model has suggested several predictions that can be tested in vivo. A tight connection was also created between the modeling and laboratory experiments. To investigate a connection between two components of the network, retinoic acid (RA) and Hoxa1, a novel laboratory experiment was performed to perturb the system. An RA soaked bead was implanted into the neural tube of a developing chick embryo and the effect of the exogenous RA was assayed with an in situ hybridization for the gene Hoxa1. The resulting expression patterns suggested that one aspect of the model design was not accurate, and based on these results the model was modified to encompass the new data, without losing the fit to the original data sets. The thesis work was therefore brought full circle, thus showing the utility of an interconnected effort: the act of constructing and using the model identified interesting biology questions, and the answer to one of those questions was used to enhance the model.
Files	Filename       Size       Approximate Download Time (Hours:Minutes:Seconds)     28.8 Modem   56K Modem   ISDN (64 Kb)   ISDN (128 Kb)   Higher-speed Access    Kastner_Abstract.pdf 59.07 Kb 00:00:16 00:00:08 00:00:07 00:00:03 < 00:00:01   Kastner_Appendix.pdf 1.81 Mb 00:08:22 00:04:18 00:03:46 00:01:53 00:00:09   Kastner_Chapter1.pdf 290.50 Kb 00:01:20 00:00:41 00:00:36 00:00:18 00:00:01   Kastner_Chapter2.pdf 637.03 Kb 00:02:56 00:01:31 00:01:19 00:00:39 00:00:03   Kastner_Chapter3.pdf 2.65 Mb 00:12:16 00:06:18 00:05:31 00:02:45 00:00:14   Kastner_Chapter4.pdf 1.17 Mb 00:05:23 00:02:46 00:02:25 00:01:12 00:00:06   Kastner_Chapter5.pdf 79.01 Kb 00:00:21 00:00:11 00:00:09 00:00:04 < 00:00:01   Kastner_FullThesis.pdf 6.54 Mb 00:30:15 00:15:33 00:13:37 00:06:48 00:00:34